anti rpl7a Search Results


anti-rpl7a  (Absolute Biotech Inc)
90
Absolute Biotech Inc anti-rpl7a
Anti Rpl7a, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-rpl7a/product/Absolute Biotech Inc
Average 90 stars, based on 1 article reviews
anti-rpl7a - by Bioz Stars, 2026-03
90/100 stars
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anti rpl7a  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti rpl7a
Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and <t>anti‐RPL7A</t> (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.
Anti Rpl7a, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rpl7a/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
anti rpl7a - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and anti‐RPL7A (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.

Journal: Advanced Science

Article Title: Pre‐rRNA Facilitates TopBP1‐Mediated DNA Double‐Strand Break Response

doi: 10.1002/advs.202206931

Figure Lengend Snippet: Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and anti‐RPL7A (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.

Article Snippet: Detailed information of antibodies used in this study: anti‐Flag (Sigma, Rabbit, F7425), anti‐TopBP1 (Santa Cruz, Mouse, sc‐271043; BETHYL, Rabbit, A300‐111A), anti‐γH2AX (Novus, Rabbit, NB100‐384; CST, Rabbit, 9718), anti‐SCP3 (Santa Cruz, Mouse, sc‐74569; Novus, Rabbit, NB300‐232), anti‐rRNA (Novus, Mouse, NB100‐662), anti‐RPL7A (ABclonal, Rabbit, A13713), anti‐RPS3 (ABclonal, Rabbit, A2533), anti‐FBL (CST, Rabbit, 2639S; Santa cruz, Mouse, sc‐166001), IgG (Rabbit, CST, 2729), IgG (Mouse, CST, 3420), anti‐ATR (BETHYL, Rabbit, A300‐137A), anti‐Chk1 (Novus, Rabbit, NB100‐464), anti‐pATR (T1989) (GeneTex, Rabbit, GTX128145), anti‐pChk1 (Ser345) (CST, Rabbit, 2348), anti‐GAPDH (CST, Mouse, 97 166), anti‐pH3 (Ser10) (CST, Rabbit, 9701), anti‐Puromycin (Millipore, Mouse, MABE343), anti‐GAPDH (HUABIO, Rabbit, ET1601‐4), anti‐Histone H3 (CST, Rabbit, 4499S), anti‐ATM (GeneTex, Mouse, GTX70107), anti‐pATM (S1981) (ROCKLAND, Mouse, 200‐301‐400), anti‐pRPA (Novus, Rabbit, NBP1‐23017), anti‐Rad9 (Santa Cruz, Mouse, sc‐74464), anti‐RAD51 (abcam, Rabbit, ab63801).

Techniques: Marker, Staining, Fluorescence, Two Tailed Test